
SARS-COV-2 PCR Kits
The PCR reagents have been designed to amplify low copy numbers of templates containing nucleic acid sequences typically found in SARS-CoV-2. Along with all components required for amplification, the reagents include fluorescence probes to measure amplicon formation with a suitable real time PCR instrument.
- Sampling Control
A separate non-competitive amplification system detecting human genomic DNA enables the monitoring of the sampling quality. Positive results for RNase P indicate that sampling has been carried out with sufficient thoroughness, and thus allows reducing false negative results due to insufficient sample collection. - Dual target design
Our assay design enables high sensitivity and robustness by amplification and detection of two independent SARS-CoV-2 genomic sequences. - Amplicon contamination control
The use of Uracil-N-Glycosylase (UNG) and dUTP is a sophisticated and acknowledged concept to avoid amplicon contamination in the PCR laboratory. Processing a lot of positive samples through PCR without contamination control could pose a serious risk to laboratory workflows.
For more information please contact us.
Description
SARS CoV-2 PCR Reagent Kit
- The kit contains primer/probes and an enzyme-mix to amplify the ORF1ab and N-Gene and the human RNase P sequences.
- It can be used in combination with commercial extraction methods.
- It can be used with a variety of modern commercial real time thermo cyclers.
- The 25 µl PCR ensures amplification of low copy numbers of templates.
- PCR product cross contaminations are suppressed by the use of UNG.
Specifications
Cycler | QuantStudio 5TM | LightCycler® 480 II |
LoD95 | 19 cp/reaction | 13 cp/reaction |
Confidence Interval | 14 – 31 | 11 – 20 |
Specificity
The SARS-CoV-2 PCR Reagent Kit has been tested against a wide range of potentially interfering viruses without any cross reactivity.
Order information
Product | Order number | # of reactions |
SARS-CoV-2 PCR Reagent Kit | R2K-288-30 | 8’640 (288 x 30) |
Regulatory notes
Intended Use
The SARS-CoV-2 PCR Reagent Kit is designed to amplify low copy numbers of templates containing nucleic acid sequences typically found in SARS-CoV-2. Along with all components required for amplification, the reagents include fluorescence probes to measure the formation of PCR products with a suitable real-time PCR instrument.
For research use only (RUO)! Not for use in diagnostic procedures.
PoET Instrument
Explore next level NAT testing
Accesories
Many Accessories available
Contact
For detailed information
Doc. HO 000219