High performance PCR reagents for SARS-CoV-2

The PCR reagents have been designed to support screening laboratories during the Corona crisis.

SARS-CoV-2 PCR reagent kit

The PCR reagents have been designed to amplify low copy numbers of templates containing nucleic acid sequences typically found in SARS-CoV-2. Along with all components required for amplification, the reagents include fluorescence probes to measure amplicon formation with a suitable real time PCR instrument.

  • Sampling Control
    A separate non-competitive amplification system detecting human genomic DNA enables the monitoring of the sampling quality. Positive results for RNase P indicate that sampling has been carried out with sufficient thoroughness, and thus allows reducing false negative results due to insufficient sample collection.
  • Dual target design
    Our assay design enables high sensitivity and robustness by amplification and detection of two independent SARS-CoV-2 genomic sequences.
  • Amplicon contamination control
    The use of Uracil-N-Glycosylase (UNG) and dUTP is a sophisticated and acknowledged concept to avoid amplicon contamination in the PCR laboratory. Processing a lot of positive samples through PCR without contamination control could pose a serious risk to laboratory workflows.

For more information please contact us.

SARS CoV-2 PCR Reagent Kit

  • The kit contains primer/probes and an enzyme-mix to amplify the ORF1ab and N-Gene and the human RNase P sequences.
  • It can be used in combination with commercial extraction methods.
  • It can be used with a variety of modern commercial real time thermo cyclers.
  • The 25 µl PCR ensures amplification of low copy numbers of templates.
  • PCR product cross contaminations are suppressed by the use of UNG.
CyclerQuantStudio 5TMLightCycler® 480 II
LoD9519 cp/reaction13 cp/reaction
Confidence Interval14 – 3111 – 20

Specificity

The SARS-CoV-2 PCR Reagent Kit has been tested against a wide range of potentially interfering viruses without any cross reactivity.

For details please refer to the product information document.

Product
Order number
# of reactions
SARS-CoV-2 PCR Reagent KitR2K-288-308’640 (288 x 30)

Intended Use

The SARS-CoV-2 PCR Reagent Kit is designed to amplify low copy numbers of templates containing nucleic acid sequences typically found in SARS-CoV-2. Along with all components required for amplification, the reagents include fluorescence probes to measure the formation of PCR products with a suitable real-time PCR instrument.

For research use only (RUO)! Not for use in diagnostic procedures.

Portfolio

Quick links to our assay portfolio.

Doc. HO-4023

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