West Nile Virus

The WNV PCR Kit is designed for the qualitative detection of West Nile virus (WNV) RNA in human EDTA plasma obtained from blood donations, and the confirmation of positive screening results.

West Nile Virus

The WNV PCR Kit is a ready-to-use test for the detection of West Nile virus RNA by reverse transcription (RT) and real-time polymerase chain reaction (real-time PCR) in the Roche LightCycler® 480 II real-time PCR system.

Sensitivity: 95% Limit of Detection (95% LOD) for Roche LightCycler® 480
Analyte (PCR version)
Reference material
Sample pools (n = 8)
sample volume 1 ml each
Confirmatory samples
sample volume 1 ml
WNV (v3)
Istituto Superiore di Sanità, Rome; Lot: ISS 0410
22.2 cop/ml15.4 cop/ml
Sensitivity: 95% Limit of Detection (95% LOD) for Roche LightCycler® 480
Analyte (PCR version)
Reference material
Sample pools from 0.1 – 9.6 ml plasma
Examples:
pools of up to 96, sample input 100 µl
pools of up to 48, sample input 200 µl
WNV (v3)
Istituto Superiore di Sanità, Rome; Lot: ISS 0410
21.2 cop/ml
Specificity of WNV for Roche LightCycler® 480 (EDTA plasma)
Sample type tested
Specificity
Pool of 8100%
Pool of 7100%
Pool of 6100%
Pool of 5100%
Pool of 4100%
Pool of 3100%
Pool of 2100%
Confirmatory samples100%

For details please refer to the product information document.

WNV PCR Kit
WNV PCR Kit (version 1.2)
GFE order number
Number of reactions per kit
packing unit = 20 kits
032-24-20
24
Kit components:
Basismix v2
Oligomix WNV v3
PCR version

PCR version 3
Label
BM v2
O_W v3

Intended Use

The WNV PCR Kit is designed for the qualitative detection of West Nile virus (WNV) RNA in human EDTA plasma obtained from blood donations.

The WNV PCR Kit is intended for screening human plasma samples from individual donors or pooled human plasma samples of up to 9.6 ml total plasma volume and for the confirmation of human plasma samples from individual donors tested as WNV positive in screening in an individual test process.

The WNV PCR Kit is intended for use with the Roche LightCycler® 480 II real-time PCR system in conjunction with the GFE autoX-System (in the semi-automated or fully automated version). The kit must only be used with the appropriate accessory kits supplied by GFE.

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