West Nile Virus
The WNV PCR Kit is designed for the qualitative detection of West Nile virus (WNV) RNA in human EDTA plasma obtained from blood donations, and the confirmation of positive screening results.
West Nile Virus
The WNV PCR Kit is a ready-to-use test for the detection of West Nile virus RNA by reverse transcription (RT) and real-time polymerase chain reaction (real-time PCR) in the Roche LightCycler® 480 II real-time PCR system.
| Analyte (PCR version) Reference material | Sample pools (n = 8) sample volume 1 ml each | Confirmatory samples sample volume 1 ml |
| WNV (v3) Istituto Superiore di Sanità, Rome; Lot: ISS 0410 | 22.2 cop/ml | 15.4 cop/ml |
| Analyte (PCR version) Reference material | Sample pools from 0.1 – 9.6 ml plasma Examples: pools of up to 96, sample input 100 µl pools of up to 48, sample input 200 µl |
| WNV (v3) Istituto Superiore di Sanità, Rome; Lot: ISS 0410 | 21.2 cop/ml |
| Sample type tested | Specificity |
| Pool of 8 | 100% |
| Pool of 7 | 100% |
| Pool of 6 | 100% |
| Pool of 5 | 100% |
| Pool of 4 | 100% |
| Pool of 3 | 100% |
| Pool of 2 | 100% |
| Confirmatory samples | 100% |
For details please refer to the product information document.
| WNV PCR Kit (version 1.2) GFE order number Number of reactions per kit | packing unit = 20 kits 032-24-20 24 | |
| Kit components: Basismix v2 Oligomix WNV v3 | PCR version – PCR version 3 | Label BM v2 O_W v3 |
Intended Use
The WNV PCR Kit is designed for the qualitative detection of West Nile virus (WNV) RNA in human EDTA plasma obtained from blood donations.
The WNV PCR Kit is intended for screening human plasma samples from individual donors or pooled human plasma samples of up to 9.6 ml total plasma volume and for the confirmation of human plasma samples from individual donors tested as WNV positive in screening in an individual test process.
The WNV PCR Kit is intended for use with the Roche LightCycler® 480 II real-time PCR system in conjunction with the GFE autoX-System (in the semi-automated or fully automated version). The kit must only be used with the appropriate accessory kits supplied by GFE.
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