PCR Negative Control

The NTC Kit serves as a negative control for PCR tests.

No Template Control

The PCR reagents used (Basismix and Oligomix) can be checked for contaminating nucleic acid sequences by the use of the NTC Kit. It is used instead of nucleic acid extracts as input for the PCR reaction (No Template Control). This also allows to detect potential contaminations by the Internal Control (IC).

The NTC Kit monitors possible contaminations of PCR reagents and should provide negative results. In case of a positive result of the No Template Control, the whole run is considered as invalid for the respective parameter.

For details please refer to the product information document.

NTC Kit
NTC Kit (version 1.0)
GFE order number
packing unit = 30 kits
013-180-30
Kit components:
Aqua NTC (v1)
Volume [µl]
350
Label
NTC

Intended Use

The NTC (“No Template Control“) Kit is a control kit for the Nucleic acid Amplification Technique (NAT) tests of GFE. It is designed as a negative control (no template control, NTC) for Real-Time PCR virus detection in human EDTA plasma, which has been donated in the context of blood banking. The control function ranges from PCR setup to the amplification of viral nucleic acids and result evaluation.

The NTC Kit is intended for use with the Applied BiosystemsTM 7500 Real-Time PCR System (Applied Biosystems by Life Technologies GmbH) or the Roche LightCycler® 480 II Real-Time PCR System in conjunction with the GFE autoX-System and the respective GFE PCR and accessory kits.

The NTC Kit is also intended for use with the Roche LightCycler® 480 II Real-Time PCR System in conjunction with the QIAamp® MinElute® Virus Spin Kit (QIAGEN GmbH) for nucleic acid extraction and the respective GFE PCR and control kits.

Doc. HO-4021

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